IG Live: with Nicholas Rust of Rusty Exotics
Introduction to Orchid Seed Propagation
This conversation with Nicholas Rust of Rusty Exotics explores the methods and challenges of orchid propagation with a home lab set up. Our peek into plant propagation through “flasking” offers insight into the kind of set up required for a home tissue culture lab.
The Nature of Orchid Seeds
Orchid seeds are unique because of their small size and lack of stored nutrients. Unlike typical seeds, they have a protective layer and depend on fungi for nutrients in nature in order to germinate.
Key Facts About Orchid Seeds:
Size and Quantity:
Orchid seeds are tiny, almost dust-like.
A single pod can contain millions of these seeds, unlike other plants which may only have a few hundred.
Germination Process:
These seeds rely on specific fungi for nutrients.
The fungi provide what is needed for germination into a stage known as protocorm.
Propagation Process Overview
In a lab, orchid propagation involves creating a controlled environment that mimics natural conditions. Agar plates with added sugars and nutrients provide the necessary setting for growth, in the absence of the fungi in nature.
Steps in the Process:
Maintaining a Sterile Environment:
Propagation requires a clean setting to avoid contamination.
Activated charcoal in agar prevents harmful compounds from affecting growth.
2. Growth Stages:
Seeds develop into protocorms and are then moved to new mediums.
These grow into young plants under fairly standard light (artificial light) and temperature (room temp) settings.
Setting Up Your Own Lab
For those interested in propagation, setting up a home lab can be rewarding. Here’s a simple guide to start:
Essential Equipment:
A homemade or professional flow hood for a sterile workspace.
A pressure cooker for sterilizing tools and equipment.
Jars with lids (flasks)
Supplies and tools for growing like agar medium, activated charcoal, alcohol, long tweezers / forceps.
Getting Started:
Create a sterile area for work.
Learn about the processes of replating and plant care.
Important Tips:
Begin Simply: Start with easy plant species and gradually expand.
Stay Educated: Use resources and community assistance for support.
Accept Trial and Error: We learned from Nicholas that it can take a long time to learn lab propagation for a given plant type.